A Single Multiomics Transistor for Electronic Detection of SARS‐Cov2 Variants Antigen and Viral RNA Without Amplification

The SARS-CoV-2 pandemic caused a public health crisis throughout the world and highlighted need for rapid sensitive testing as countermeasure. A specific biosensor platform is developed detection of antigen RNA SARS-CoV-2, its variant (B1.1.529). demonstrated combines unique protein catalyzed capture bioreceptors (PCCs) chimeric (RNA-DNA) probe using LwaCas13a collateral cleavage activity atop graphene field effect transistors (gFETs). reported able to differentiate unprocessed 104 pfu m−1 samples from Influenza Rhinovirus. limit (LOD) calculated 103 in buffer PFU mL−1 10% saliva, while LOD ≈65 am viral isolate without amplification. To provide high reliability detection, role internal external factors with respect gate voltage further analyzed by Principal Component Analysis (PCA). Based on PCA analysis, authors are classify pathogen positive or negative (Y > 0: Positive pathogen, Y < Negative pathogen). can be quickly adapted multi-omics multiplexed diagnosis continuously evolving biothreats global pandemics.